Reporter
Part:BBa_K389403:Design
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-10-11)
Sensitivity tuner + luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1322
Illegal NgoMIV site found at 2666
Illegal NgoMIV site found at 2687
Illegal AgeI site found at 845
Illegal AgeI site found at 957
Illegal AgeI site found at 1159
Illegal AgeI site found at 2390 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2572
Design Notes
arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part BBa_I746390
Source
- luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
- sensitivity tuner part consists inter alia of
- delta activator from phage phiR73 (BBa_I746352)
- PF promoter from phage P2 (BBa_I746360)
References
[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]